Conventional PCR versus Culture Method to Detect Common Fungal Pathogens in Patients with Respiratory Diseases
نویسندگان
چکیده
The study aimed to assess the frequency of invasive fungal infection in patients with respiratory diseases by conventional and molecular methods. This included 117 Broncho alveolar lavage (BAL) samples were collected from disease (79 male 38 female) ages ranged between (20-80) years, who attended Medicine Baghdad Teaching hospital AL-Emamain AL-Khadhymian Medical City, during period September 2019 April 2020. results PCR versus culture methods this showed that out infections 30(25.6 %) detected method, while 24(20.5%) technique, most commonly diagnosed pathogenic fungi is Candida spp. followed Aspergillus By considering method as a gold standard against show sensitivity specificity (86.6%) (100%) respectively.
منابع مشابه
Development of a Multiplex PCR Method to Detect Fungal Pathogens for Quarantine on Exported Cacti.
Major diseases in grafted cacti have been reported and Fusarium oxysporum, Bipolaris cactivora, Phytophthora spp. and Collectotrichum spp. are known as causal pathogens. These pathogens can lead to plant death after infection. Therefore, some European countries have quarantined imported cacti that are infected with specific fungal pathogens. Consequently, we developed PCR detection methods to i...
متن کاملSilver nanoparticles, a potential alternative to conventional anti-fungal agents to fungal pathogens affecting crop plants
Metallic silver nanoparticles have been reduced from silver nitrate by employing the extracellular enzymatic machinery of edible White button Mushroom (Agaricus bisporus). The physical properties of these particles, size and shapes have been determined using techniques like TEM, FTIR and XRD and were reported in our earlier report. But, what stands of paramount importance in the presen...
متن کاملSilver nanoparticles, a potential alternative to conventional anti-fungal agents to fungal pathogens affecting crop plants
Metallic silver nanoparticles have been reduced from silver nitrate by employing the extracellular enzymatic machinery of edible White button Mushroom (Agaricus bisporus). The physical properties of these particles, size and shapes have been determined using techniques like TEM, FTIR and XRD and were reported in our earlier report. But, what stands of paramount importance in the presen...
متن کاملDetection of fungi by conventional methods and semi-nested PCR in patients with presumed fungal keratitis
Background and Purpose: Fungal keratitis is a suppurative, ulcerative, and sight-threatening infection of the cornea that sometimes leads to blindness. The aims of this study were: recuperating facilities for laboratory diagnosis, determining the causative microorganisms, and comparing conventional laboratory diagnostic tools and semi-nested PCR. Materials and Methods: Sampling was conducted in...
متن کاملfungal pathogens associated with grapevine trunk diseases in iran
during 2004–2007 various own rooted vineyards were inspected to study the fungi associated with vine trunk diseases in iran. samples from declining vines showing yellowing and reduced growth and different symptoms in wood, including browning of the wood, necrosis, brown and black streaking and white rot were collected. fungal isolations were made from affected tissues onto malt extract agar (me...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biomedicine and chemical sciences
سال: 2022
ISSN: ['2790-296X']
DOI: https://doi.org/10.48112/bcs.v1i4.235